Hormones in karst springs

Grad student Jim Berglund, undergrad Rachel Crowley, and I designed a sampling program for hormones in karst springs near Valley Creek just east of Philadelphia. We sampled springs varying distances from septic systems and roads, as well as in Valley Forge National Park (after getting a park sampling permit).   Lab analysis required the help of  colleague Jack Gordon in the Pharmacy School and learning how to use Elissa kits (pictured below).  Lots of duplicate measurements provided confidence in the low concentrations we detected.

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