The lab has a new accepted publication:
Hamsher, S.E., C.Y.T Sung and R.W. Sanders. on line Dec 2017. Effects of temperature and photorepair radiation on a marine ciliate exposed to UV-B radiation. J. Eukaryotic Microbiology [
Bob Sanders participated in writing a paper on the biogeography of protists that acquire the ability to photosynthesize via symbiosis or kleptoplasty.
Leles, S.G., A. Mitra, K.J. Flynn, D.K. Stoecker, P.J. Hansen, A. Calbet, G B. McManus, R.W. Sanders, D.A. Caron, F. Not, G. M. Hallegraeff, P. Pitta, J.A. Raven, M.D. Johnson, P.M. Glibert, S. Våge. 2017. Oceanic protists with different forms of acquired phototrophy display diverse biogeographies and abundance. 2017. Proceedings of the Royal Society B 284: 20170664. [doi: 10.1098/rspb.2017.0664]
A paper based on a presentation given by Steffi Moorthi at a “Symposium on Aquatic Microbial Ecology (SAME)” was recently published. Moorthi, S.D., R. Ptacnik, R.W. Sanders, M. Busch, R. Fischer, H. Hillebrand. 2017. The functional role of planktonic mixotrophs in altering seston stoichiometry. Aquatic Microbial Ecology 79: 235-245. [doi:10.3354/ame01832]
Sarah Hamsher, previously at the University of Colorado, Boulder has joined the lab. Her research interests revolve around the evolutionary relationships of eukaryotic microorganisms – especially diatoms.
Research Faculty Position Has Been Filled
The Ecological Impact of Mixotrophic Algae in a Changing Arctic Climate
A 2-year non-tenure track research position is available starting as early as February 1, 2017 as part of a project investigating Arctic protists that have the ability to use both photosynthetic and heterotrophic nutrition (mixotrophs). The goals of the project are to: 1) study the physiology of different algal groups that have been identified as mixotrophic and occur in the Arctic seas; this includes an examination of how changes in irradiance, temperature and dissolved nutrients affect photosynthesis versus bacterivory; 2) evaluate differential gene expression using RNAseq methods in cultures where grazing is high versus low for a subset of species from each of the major groups; and 3) relate grazing rates to the abundance of the Arctic species determined using qPCR and environmental DNA samples previously collected throughout the Arctic to estimate grazing impact by mixotrophs in the polar ecosystem.
The researcher will join a team lead by Robert Sanders (Temple University) and Rebecca Gast (Woods Hole Oceanographic Institution), and will work with Arctic cultures in laboratory-based experiments. Basic qualifications are a PhD in aquatic ecology, biological oceanography or a related field. Experience in protistan/ phytoplankton biology, including culturing, is expected. Previous training in molecular techniques and/or bioinformatics is highly preferred, but not required. The position will be based at Temple University in Philadelphia PA, but the researcher will also work closely with the Gast laboratory in Woods Hole MA. To apply please send an application letter, curriculum vitae and names of at least three professional references to Bob Sanders. Review of applications will begin immediately and continue until the position is filled. Temple University is an equal opportunity, equal access, affirmative action employer committed to achieving a diverse community (AA, EOE, m/f/d/v).
Collaborative Research: The ecological impact of mixotrophic algae in a changing Arctic marine climate. National Science Foundation, Arctic Natural Sciences. Co-PIs: R.J. Gast (Woods Hole Oceanographic Institution) and R.W. Sanders. 2016-2019.
Another 1st author paper for Sarah:
Princiotta, S.D. and R.W. Sanders. 2017. Heterotrophic and mixotrophic nanoflagellates in a mesotrophic lake: abundance and grazing impacts across season and depth. Limnology & Oceanography 62: 632-644.
Brian Smith, who was an undergraduate volunteer in the lab, is starting in the MS program in Marine Science & Technology at University of Massachusetts in September. Congratulations to Brian !
Sarah has accepted a position a as Director of Research and Education at the Lacawac Sanctuary & Biological Field Station in Lake Ariel, Pennsylvania. She starts July 1. Congratulations Sarah!
May 6, 2016 – Drs. Sanders & DeVaul Princiotta
Sarah gave a talk at the annual meeting of the Northeast Algal Society in April 2016 (Vertical distribution of mixotrophic nanoflagellates in a mesotrophic lake; S. DeVaul & R. Sanders). Bob presented a poster of Zaid’s last dissertation chapter (Competitive interactions of two Antarctic mixotrophs with either phototrophic or phagotrophic specialists; Z. McKie-Krisberg & R. Sanders).
The last bit of Erin’s Ph.D. work is: Graham, E.R. & Sanders, R.W. 1916. Species-specific photosynthetic responses of symbiotic zoanthids to thermal stress and ocean acidification. Marine Ecology 37: 442-458. Here’s a link.
Zaid has been awarded an NSF Postdoctoral Research Grant entitled: “A Systems Approach to Investigations of Light Independent C4 Type Carbon Fixation in Green Algae.”
Sarah presented some of her Ph.D. work on mixotrophy at the winter meeting (February 21-26) of the Association for the Sciences of Limnology and Oceanography (ASLO) . Bob presented work from his last ocean voyage in the Antarctic with Rebecca Gast of Woods Hole Oceanographic Institution. There are also a number of graduate students from the Cordes lab that presented at the meeting.
Temperature-dependent phagotrophy and phototrophy in a mixotrophic chrysophyte by Sarah DeVaul Princiotta, Brian T. Smith and Robert W. Sanders has been published in the Journal of Phycology (52:432-440) and can be found here.
Defining planktonic protist functional groups on mechanisms for energy and nutrient acquisition: incorporation of diverse mixotrophic strategies by Aditee Mitra and 22 co-authors (including R.W. Sanders) was published in Protist (167:106-120) and is available online at http://www.sciencedirect.com/science/article/pii/S1434461016000043
At the 2015 Annual Meeting of the Phycological Society of America, which happened to be in Philadelphia this year, Sarah was awarded the Bold Award for the best graduate student talk during the conference. Her talk was entitled “Striking a Balance between Phototrophy and Heterotrophy in the Mixotrophic Chrysophyte Dinobryon sp.” Congrats to Sarah!
The lab will have three presentations at the Phycological Society of America annual meeting that is taking place in Philadelphia this month.
Sarah DeVaul has an oral presentation entitled: Striking a balance between phototrophy and heterotrophy in the mixotrophic chrysophyte Dinobryon sp.;
Grier Sellers talk with Rebecca Gast (Woods Hole Oceanographic Institution is entitled: Dark survival and recovery of a foreign organelle-retaining dinoflagellate and its haptophyte prey and plastid source following a simulated austral winter
and Bob Sanders will present a poster by Zaid McKie-Krisberg, Rebecca Gast and Bob Sanders entitled: Gene expression and gene ontology in two species of mixotrophic Antarctic phytoplankton.
Sanders, R.W., S.L. Cooke, J.M. Fischer, S.B. Fey, A.W. Heinze*, W.H. Jeffrey, A.L. Macaluso*, R.E. Moeller, D.P. Morris, P.J. Neale, M. Olson, J.D. Pakulski, J.A. Porter, D.M. Schoener*, C.E. Williamson. 2015. Shifts in microbial food web structure and productivity after additions of naturally occurring dissolved organic matter: results from large-scale lacustrine mesocosms. Limnology & Oceanography. 60: 2130-2144. DOI: 10.1002/lno.10159
Cooke, S.L., J.M. Fischer, K. Kessler, Craig E. Williamson, R.W. Sanders, D.P. Morris, J.A. Porter, W.H. Jeffrey, S.B. DeVaul*, J.D. Pakulski. 2015. Direct and indirect effects of additions of chromophoric dissolved organic matter on zooplankton during large-scale mesocosm experiments in an oligotrophic lake. Freshwater Biology. 60:2362-2378. DOI:10.1111/fwb.12663
*Current or former graduate students in the lab.
Erin’s final paper from her Ph.D. work was published: Graham, E.R., A. Parekh, R.K. Devassy and R.W. Sanders. 2015. Carbonic anhydrase activity changes in response to increased temperature and pCO2 in Symbiodinium-zoanthid associations.Journal of Experimental Marine Biology and Ecology 473:218-226. Here’s a link.
Amy Parekh and Roni Devassy were undergraduate researchers in the lab.
Congrats to them all!
McKie-Krisberg, Z.M., R.J. Gast and R.W. Sanders. 2015. Physiological responses of three species of Antarctic mixotrophic phytoflagellates to changes in light and dissolved nutrients. Microbial Ecology 70:21-29.
Abstract: Antarctic phototrophs are challenged by extreme temperatures, ice cover, nutrient limitation and prolonged periods of darkness. Yet this environment may also provide niche opportunities for phytoplankton utilizing alternative nutritional modes. Mixotrophy, the combination of photosynthesis and particle ingestion, has been proposed as a mechanism for some phytoplankton to contend with the adverse conditions of the Antarctic. We conducted feeding experiments using fluorescent bacteria-sized tracers to compare the effects of light and nutrients on bacterivory rates in three Antarctic marine photosynthetic nanoflagellates representing two evolutionary lineages: Cryptophyceae (Geminigera cryophila), and Prasinophyceae (Pyramimonas tychotreta and Mantoniella antarctica). Only G. cryophila had previously been identified as mixotrophic. We also measured photoautotrophic abilities over a range of light intensities (P vs. I) and used dark survival experiments to assess cell population dynamics in the absence of light. Feeding behavior in these three nanoflagellates was affected by either light, nutrient levels, or a combination of both factors in a species-specific manner that was not conserved by evolutionary lineage. The different responses to environmental factors by these mixotrophs supported the idea of tradeoffs in the use of phagotrophy and phototrophy for growth.