Rothberg Laboratory publications, from research at the University of Texas Medical School at San Antonio (2002-2008) and Temple University School of Medicine (2008-present). A complete list of Brad Rothberg’s publications can be found here: https://www.ncbi.nlm.nih.gov/pubmed/?term=rothberg+bs
1. Rothberg BS. (2004) Allosteric modulation of ion channels: the case of maxi-K. Science’s STKE (now called Science Signaling) 2004:pe16. http://stke.sciencemag.org/content/2004/227/pe16.long
2. Wang B, Rothberg BS, Brenner R. (2006) Mechanism of β4 subunit modulation of BK channels. Journal of General Physiology 127:449-465. http://jgp.rupress.org/content/127/4/449.long
3. Parfenova LV, Crane BM, Rothberg BS. (2006) Modulation of MthK potassium channel activity at the intracellular entrance to the pore. Journal of Biological Chemistry, 281:21131‑21138. http://www.jbc.org/content/281/30/21131.long
4. Parfenova LV, Rothberg BS. (2006) Genetic screening for functionality of bacterial potassium channel mutants using K+ uptake-deficient Escherichia coli. Methods in Molecular Biology 337:157‑165. https://link.springer.com/protocol/10.1385%2F1-59745-095-2%3A157
5. Koval OM, Fan Y, Rothberg BS. (2007) A role for the S0 transmembrane segment in voltage-dependent gating of BK channels. Journal of General Physiology, 129:209-220. http://jgp.rupress.org/content/129/3/209.long
6. Parfenova LV, Abarca-Heidemann K, Crane BM, Rothberg BS. (2007) Molecular architecture and divalent cation activation of TvoK, a prokaryotic potassium channel. Journal of Biological Chemistry, 282:24302-24309. http://www.jbc.org/content/282/33/24302.long
7. Rothberg BS. (2008) Return of the electric binding site. Journal of General Physiology. 132:487-489. http://jgp.rupress.org/content/132/5/487.long
8. Wang B, Rothberg BS, Brenner R. (2009) Mechanism of increased BK channel activation from a channel mutation that causes epilepsy. Journal of General Physiology 133:283-294. http://jgp.rupress.org/content/133/3/283.long
9. Semenova NP, Abarca-Heidemann K, Loranc E, Rothberg, BS. (2009) Bimane fluorescence scanning suggests secondary structure near the S3-S4 linker of BK channels. Journal of Biological Chemistry 284:10684-10693. http://www.jbc.org/content/284/16/10684.long
10. Pau VP, Abarca-Heidemann K, Rothberg BS. (2010) Allosteric mechanism of Ca2+-activation and H+-inhibited gating of the MthK K+ Journal of General Physiology 135:509-526. http://jgp.rupress.org/content/135/5/509
11. Thomson AS, Rothberg BS. (2010) Voltage-dependent inactivation gating at the selectivity filter of the MthK K+ Journal of General Physiology 136:569-579. http://jgp.rupress.org/content/136/5/569.long
12. Pau VP, Smith FJ, Taylor AB, Parfenova LV, Samakai E, Callaghan MM, Abarca‑Heidemann K, Hart PJ, Rothberg BS. (2011) Structure and function of multiple Ca2+-binding sites in a K+ channel regulator of K+ conductance (RCK) domain. Proceedings of the National Academy of Sciences USA 108:17684-17689. http://www.pnas.org/content/108/43/17684.long
13. Mallilankaraman K, Doonan P, Cárdenas C, Chandramoorthy HC, Müller M, Miller R, Hoffman NE, Gandhirajan RK, Molgó J, Birnbaum MJ, Rothberg BS, Mak DO, Foskett JK, Madesh M. (2012) MICU1 Is an Essential Gatekeeper for MCU-Mediated Mitochondrial Ca(2+) Uptake that Regulates Cell Survival. Cell 151:630-644. https://www.sciencedirect.com/science/article/pii/S0092867412012251?via%3Dihub
14. Smith FJ, Pau VP, Cingolani G, Rothberg BS. (2012) Crystal Structure of a Ba(2+)-Bound Gating Ring Reveals Elementary Steps in RCK Domain Activation. Structure 20:2038‑2047. https://www.sciencedirect.com/science/article/pii/S0969212612003486?via%3Dihub
15. Soboloff J, Rothberg BS, Madesh M, Gill DL. (2012) STIM proteins: dynamic calcium signal transducers. Nature Reviews Molecular Cell Biology 13:549-65. https://www.nature.com/articles/nrm3414
16. Rothberg BS. (2012) The BK channel: a vital link between cellular calcium and electrical signaling. Protein & Cell 3:883-892. https://link.springer.com/article/10.1007%2Fs13238-012-2076-8
17. Rothberg BS, Wang Y, Gill DL. (2012) Orai channel pore properties and gating by STIM: implications from the Orai crystal structure. Science Signaling 6:pe9. http://stke.sciencemag.org/content/6/267/pe9.full
18. Smith FJ, Rothberg BS. (2013) Analysis of Ca2+-binding sites in the MthK RCK domain by X‑ray crystallography. Methods in Molecular Biology 998:277-287. https://link.springer.com/protocol/10.1007%2F978-1-62703-351-0_22
19. Smith FJ, Pau VP, Cingolani G, Rothberg BS. (2013) Structural basis of allosteric interactions among Ca2+ binding sites in a K+ channel RCK domain. Nature Communications 4:2621 doi: 10.1038/ncomms3621. https://www.nature.com/articles/ncomms3621
20. Abarca-Heidemann K, Duchardt-Ferner E, Woehnert J, Rothberg BS. (2013) Isotope labeling strategies for analysis of an ion channel cytoplasmic domain by NMR spectroscopy. Methods in Molecular Biology 998:289-300. https://link.springer.com/protocol/10.1007%2F978-1-62703-351-0_23
21. Wang X, Wang Y, Zhou Y, Hendron E, Mancarella S, Andrake MD, Rothberg BS, Soboloff J, Gill DL. (2014) Distinct Orai-coupling domains in STIM1 and STIM2 define the Orai‑activating site. Nature Communications 5:3183 doi: 10.1038/ncomms4183. https://www.nature.com/articles/ncomms4183
22. Thomson AS, Heer FT, Smith FJ, Hendron E, Bernèche S, Rothberg BS. (2014) Initial steps of inactivation at the K+ channel selectivity filter. Proceedings of the National Academy of Sciences USA 111:E1713-E1722. doi: 10.1073/pnas.1317573111. http://www.pnas.org/content/111/17/E1713.long
23. Hooper R, Rothberg BS, Soboloff J. (2014) Neuronal STIMulation at rest. Science Signaling 7:pe18. http://stke.sciencemag.org/content/7/335/pe18.full
24. McManus OB, Rothberg BS. (2014) An old probe sheds new light on BK channel pore structure. Journal of General Physiology. 144:499-501. doi: 1085/jgp.201411306 http://jgp.rupress.org/content/144/6/499
25. Zhou Y, Wang X, Wang X, Loktionova NA, Cai X, Nwokonko RM, Vrana E, Wang Y, Rothberg BS, Gill DL. (2015) STIM1 dimers undergo unimolecular coupling to activate Orai1 channels. Nature Communications 6:8395. doi: 10.1038/ncomms9395. https://www.nature.com/articles/ncomms9395
26. Hooper R, Rothberg BS, Soboloff J. (2016) Sterol hindrance of Orai activation. Science Signaling 9:fs4. http://stke.sciencemag.org/content/9/418/fs4.full
27. Zhou Y, Cai X, Loktionova NA, Wang X, Nwokonko RM, Wang X, Wang Y, Rothberg BS, Trebak M, Gill DL. (2016) The STIM1 binding site nexus remotely controls Orai1 channel gating. Nature Communications 7:13725. doi: 10.1038/ncomms13725. https://www.nature.com/articles/ncomms13725
28. Giraldez T, Rothberg BS. (2017) Understanding the conformational motions of RCK gating rings. Journal of General Physiology 149:431-441. pii: jgp.201611726. doi: 10.1085/jgp.201611726. http://jgp.rupress.org/content/149/4/431.long
29. Rothberg BS. (2017) A Slick, Slack pathway through the pore. Journal of Physiology 595:2337‑2338. doi:10.1113/JP273497. https://physoc.onlinelibrary.wiley.com/doi/full/10.1113/JP273497
30. Howard RJ, Carnevale V, Delemotte L, Hellmich UA, Rothberg BS. (2018) Permeating disciplines: overcoming barriers between molecular simulations and classical structure-function approaches in biological ion transport. Biochimica et Biophysica Acta 1860:927-942. pii: S0005-2736(17)30405-4. doi: 10.1016/j.bbamem.2017.12.013. https://www.sciencedirect.com/science/article/pii/S0005273617304054?via%3Dihub
31. Li Q, Li Y, Wei H, Pan H-M, Vouga AG, Rothberg BS, Wu Y, Yan J. (2018) Molecular determinants of Ca2+ sensitivity at the intersubunit interface of the BK channel gating ring. Scientific Reports 8:509. doi: 10.1038/s41598-017-19029-8. https://www.nature.com/articles/s41598-017-19029-8
32. Rothberg BS. (2018) Charge of the Proton Channel. Biophysical Journal 114:2759-2761. doi: 10.1016/j.bpj.2018.05.008. https://www.sciencedirect.com/science/article/pii/S0006349518305848?via%3Dihub
33. Kopec W, Rothberg BS, de Groot BL. (2019) Molecular mechanism of a potassium channel gating through activation gate-selectivity filter coupling. Nature Communications 10:5366. doi: 10.1038/s41467-019-13227-w. https://www.nature.com/articles/s41467-019-13227-w
34. Rockman ME, Vouga AG, Rothberg BS. (2020) Molecular mechanism of BK channel activation by the smooth muscle relaxant NS11021. Journal of General Physiology 152:e201912506. doi: 10.1085/jgp.201912506. https://rupress.org/jgp/article/doi/10.1085/jgp.201912506
35. Suma A, Granata D, Thomson AS, Carnevale V, Rothberg BS. (2020) Polyamine blockade and binding energetics in the MthK potassium channel. Journal of General Physiology 152:e201912527. doi: 10.1085/jgp.201912527. https://rupress.org/jgp/article-lookup/doi/10.1085/jgp.201912527
36. Maduke MC, Rothberg BS. (2020) An international gathering of physiologists in Valparaiso, Chile. Journal of General Physiology 152:e202012626. doi: 10.1085/jgp.202012626. https://rupress.org/jgp/article-lookup/doi/10.1085/jgp.202012626
37. Brauchi SE, Rothberg BS. (2020) Gating and calcium-sensing mechanisms of TRPA1 channels revealed. Cell Calcium 91:102278. https://doi.org/10.1016/j.ceca.2020.102278
38. Vouga AG, Rockman ME, Yan J, Jacobson MA, Rothberg BS. (2021) State-dependent inhibition of BK channels by the opioid agonist loperamide. Journal of General Physiology 153:e202012834. https://doi.org/10.1085/jgp.202012834.