{"id":127,"date":"2019-06-10T11:34:56","date_gmt":"2019-06-10T15:34:56","guid":{"rendered":"https:\/\/sites.temple.edu\/voelzlab\/?page_id=127"},"modified":"2019-07-16T17:57:03","modified_gmt":"2019-07-16T21:57:03","slug":"cumulative-exam-june-2019","status":"publish","type":"page","link":"https:\/\/sites.temple.edu\/voelzlab\/cumulative-exam-june-2019\/","title":{"rendered":"Cumulative Exam June 2019"},"content":{"rendered":"\n<p><strong>Exam Topic<\/strong><\/p>\n\n\n\n<p><strong>Transition State Theory applied to two-state protein folding kinetics: chevron plots, phi-value analysis, and rate limiting steps<\/strong><\/p>\n\n\n\n<p>Writer: Vincent Voelz<\/p>\n\n\n\n<p>The main reading assignment to prepare for the exam is Chapters 17, 18 and 19 of the book:<\/p>\n\n\n\n<p>Fersht, A. (1998). Structure and mechanism in protein science: a guide to enzyme catalysis and protein folding. WH Freeman.&nbsp;&nbsp;<\/p>\n\n\n\n<p>You will be asked more specific questions\/problems about:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><strong>two-state folding kinetics<\/strong>(Chapter 18)<\/li><li><strong>chevron plots&nbsp;<\/strong>(Chapter 18, starting at p. 543)<\/li><li><strong>phi-value analysis&nbsp;<\/strong>(Chapter 18, starting at p. 559)<\/li><li><strong><em>m<\/em><\/strong><strong>-values and&nbsp;<\/strong><strong>\u03b2<\/strong><strong><sub>T<\/sub><\/strong><strong>-values<\/strong>(Chapter 18, starting at p. 543)<\/li><\/ul>\n\n\n\n<p>Other good phi-value references can be found in the text and citations on: <a href=\"https:\/\/en.wikipedia.org\/wiki\/Phi_value_analysis\">https:\/\/en.wikipedia.org\/wiki\/Phi_value_analysis<\/a><\/p>\n\n\n\n<p>A very good reference on measuring and analyzing protein folding kinetic data, and chevron plots can be found here:<\/p>\n\n\n\n<p>Zarrine-Afsar, A., &amp; Davidson, A. R. (2004). The analysis of protein folding kinetic data produced in protein engineering experiments. Methods, 34(1), 41\u201350.&nbsp;<a href=\"http:\/\/doi.org\/10.1016\/j.ymeth.2004.03.013\">http:\/\/doi.org\/10.1016\/j.ymeth.2004.03.013<\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Solution Key<\/h2>\n\n\n\n<div class=\"wp-block-file\"><a href=\"https:\/\/sites.temple.edu\/voelzlab\/files\/2019\/07\/solution-cume-Jun2019.pdf\">solution-cume-Jun2019<\/a><a href=\"https:\/\/sites.temple.edu\/voelzlab\/files\/2019\/07\/solution-cume-Jun2019.pdf\" class=\"wp-block-file__button\" download>Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Exam Topic Transition State Theory applied to two-state protein folding kinetics: chevron plots, phi-value analysis, and rate limiting steps Writer: Vincent Voelz The main reading assignment to prepare for the exam is Chapters 17, 18 and 19 of the book: Fersht, A. (1998). Structure and mechanism in protein science: a guide to enzyme catalysis and &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/sites.temple.edu\/voelzlab\/cumulative-exam-june-2019\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Cumulative Exam June 2019&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1258,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-127","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.temple.edu\/voelzlab\/wp-json\/wp\/v2\/pages\/127","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.temple.edu\/voelzlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.temple.edu\/voelzlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.temple.edu\/voelzlab\/wp-json\/wp\/v2\/users\/1258"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.temple.edu\/voelzlab\/wp-json\/wp\/v2\/comments?post=127"}],"version-history":[{"count":0,"href":"https:\/\/sites.temple.edu\/voelzlab\/wp-json\/wp\/v2\/pages\/127\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.temple.edu\/voelzlab\/wp-json\/wp\/v2\/media?parent=127"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}