{"id":123,"date":"2024-10-24T22:22:38","date_gmt":"2024-10-25T02:22:38","guid":{"rendered":"https:\/\/sites.temple.edu\/chemdemo\/?p=123"},"modified":"2024-10-25T16:22:01","modified_gmt":"2024-10-25T20:22:01","slug":"photosynthesis","status":"publish","type":"post","link":"https:\/\/sites.temple.edu\/chemdemo\/2024\/10\/24\/photosynthesis\/","title":{"rendered":"Photosynthesis"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Prepare Chromatography Solution<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>Materials:<\/strong><\/li>\n<\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Fresh kale: 10 g<\/li>\n\n\n\n<li>Fresh spinach: 10 g<\/li>\n\n\n\n<li>Carrot: 1\/2<\/li>\n\n\n\n<li>Red bell pepper: 1\/2<\/li>\n\n\n\n<li>Solvent mixture: Acetone:Petroleum ether (20:80)<\/li>\n<\/ul>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>Procedure:<\/strong><\/li>\n<\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Extract a ground mixture of the fresh ingredients using the acetone:petroleum ether mixture.<\/li>\n\n\n\n<li>Dry the extract over anhydrous sodium sulfate or anhydrous magnesium sulfate.<\/li>\n\n\n\n<li>Reduce the volume of the solution under a stream of air.<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Prepare Chromatography Column<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>Column Setup:<\/strong><\/li>\n<\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Place a cotton plug at the end of a chromatography column.<\/li>\n\n\n\n<li>Dampen the cotton plug with pet ether.<\/li>\n\n\n\n<li>Pour a 2 cm layer of sand over the cotton plug.<\/li>\n<\/ul>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>Fill the Column:<\/strong><\/li>\n<\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Make a slurry of silica and pet ether.<\/li>\n\n\n\n<li>Slowly fill the column with the silica slurry, ensuring no air bubbles are present.<\/li>\n\n\n\n<li>Top the slurry with another 2 cm layer of sand.<\/li>\n<\/ul>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>Elution:<\/strong><\/li>\n<\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Drain pet ether until about 1 cm covers the sand on top.<\/li>\n\n\n\n<li>Using a Pasteur pipette, add the prepared mixture to the top of the column, trying to evenly disperse the layer of solution to achieve nice bands.<\/li>\n\n\n\n<li>Elute first with acetone for the green bands, then with pet ether for the yellow bands.<\/li>\n<\/ul>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Prepare Chromatography Solution Prepare Chromatography Column<\/p>\n","protected":false},"author":36673,"featured_media":0,"comment_status":"open","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-123","post","type-post","status-publish","format-standard","hentry","category-demonstrations","entry"],"_links":{"self":[{"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/posts\/123","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/users\/36673"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/comments?post=123"}],"version-history":[{"count":2,"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/posts\/123\/revisions"}],"predecessor-version":[{"id":126,"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/posts\/123\/revisions\/126"}],"wp:attachment":[{"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/media?parent=123"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/categories?post=123"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.temple.edu\/chemdemo\/wp-json\/wp\/v2\/tags?post=123"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}