Temple University, Department of Mechanical Engineering
Metastatic Tumor Cells 1
Highly metastatic MDA-MB-231 tumor cells reduced endothelial cell adherens junction (VEcadherin) molecule expression. When cultured without the presence of tumor cells, adherens junctions were fully established between adjacent cells. When co-cultured with MDA-MB-231 tumor cells, VE-cadherin was partially expressed between adjacent cells, while no change in VE-cadherin expression was observed when HBTAEC were co-cultured with MCF-7 tumor cells. Cell adherens junction molecule was stained with VEcadherein antibody (red); cell F-actin microfilament was stained with phalloidin (green); cell nucleus was counterstained with Hoechst 33342 (blue).
Highly metastatic MDA-MB-231 tumor cells reduced endothelial cell adherens junction (VEcadherin) molecule expression. When cultured without the presence of tumor cells, adherens junctions were fully established between adjacent cells. When co-cultured with MDA-MB-231 tumor cells, VE-cadherin was partially expressed between adjacent cells, while no change in VE-cadherin expression was observed when HBTAEC were co-cultured with MCF-7 tumor cells. Cell adherens junction molecule was stained with VEcadherein antibody (red); cell F-actin microfilament was stained with phalloidin (green); cell nucleus was counterstained with Hoechst 33342 (blue).