Tumor conditioned media (TCM) (48 h treatment) from highly metastatic MDA-MB-231 tumor cells
increased liposome extravasation into the tumor compartment as indicated by the ratio of fluorescent liposome
intensity in the tumor compartment to the vascular compartment (panel A). Liposomes extravasated more
after MDA-MB-231 conditioned media or TNF-α treatment, but were not affected by TCM obtained from nonmetastatic
MCF-7 tumor cells (panels B–E). Data are presented as mean ± SEM (n = 3). *Significant difference
by ANOVA.